Anti-HDGF [H3]

Catalogue Number: AB02457-10.0-ABA

Manufacturer:Vector Laboratories, Inc (ABA)
Type:Recombinant Monoclonal
Alias:Hepatoma-derived growth factor; High mobility group protein 1-like 2; HMG-1L2
Shipping Condition:Blue Ice
Unit(s): 200 ug
Host name: Human
Clone: H3
Isotype: IgG1
Immunogen: The original antibody was raised by immunizing mice with full-length recombinant HDGF. This antibody binds a different epitope on HDGF than antibody C1.
Application: IF, IP, WB, IHC, NT

Additional Text

Gene Name

HDGF

Gene ID

3068

Uniprot ID

P51858

Purification

Purified

Antibody Clonality

Recombinant Monoclonal

Storage Note

Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.

Application Notes

This antibody was used for immunoprecipitation on total cell lysates from H460 NSCLC cells (US20070243191). The neutralizing ability of this antibody was tested on A549. The result was a significant decrease in tumor size (US20070243191). This antibody was fluorescently labelled and injected into nude mice to test the antibody distribution. The antibodies evenly distributed in the animals including the tumors. This antibody entered into the cytoplasmic compartment of some tumor cells (US20070243191). Immunohistochemistry with this antibody was performed on tissues from NSCLC patients (US20070243191). Western blot was performed on HDGF protein using this antibody (Ren et al, 2009;pmid:19435872). ELISA was performed on the HDGF protein using this antibody (US20070243191).

Short Description

This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.