Anti-Thomsen-nouveaupolysaccharide A1 [KT-IgM-8]
Catalogue Number: AB02511-10.3-ABA
| Manufacturer: | Vector Laboratories, Inc (ABA) |
| Type: | Recombinant Monoclonal |
| Alias: | Tn-PS A1; Thomsen-nouveau (Tn); Tn antigen; Tn saccharide; N-acetyl Galactosamine; Alpha-GalNAc; Alpha GalpNAc; GalNAc; GalNAc-alpha; N-acetyl-alpha-D-galactosamine; Blood group A antigen |
| Shipping Condition: | Blue Ice |
| Unit(s): | 0.1 mg |
| Host name: | Human |
| Clone: | KT-IgM-8 |
| Isotype: | IgG1 |
| Immunogen: | The original antibody was generated by immunizing C57BL/6 mice against Tn-PS A1. |
| Application: | ELISA, FC, InVitroA, InVivoA, Cytox |
Additional Text
Purification
Purified
Antibody Clonality
Recombinant Monoclonal
Storage Note
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Application Notes
The specificity of the original format of the antibody was confirmed by ELISA analysis. The ability of the antibody to bind cancer cells expressing the Tn antigen on the cell surface (MCF-7 and HCT-116) was evaluated by by FC. The antibody demonstrated the ability to bind both MCF-7 and HCT-116 tumor cells at 30 µg/mL with a shift in fluorescence of 49% in both cell lines. The antibody demonstrated in vitro tumor killing using an LDH assay. The assay measured that the antibody-induced complement-dependent cytotoxicity was 30%. In vivo passive immunotherapy approach using an MCF-7 cell line-derived xenograft model was evaluated. The antibody demonstrated approximately 40% reduction in tumor growth in the MCF-7 model (Trabbic et al., 2018; PMID: 30030557).
Short Description
This chimeric human antibody was made using the variable domain sequences of the original Mouse IgM format, for improved compatibility with existing reagents, assays and techniques.
AB02511-10.3 Datasheet
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