Anti-FGRI [M19B2]
Catalogue Number: AB02531-6.1-BT-ABA
| Manufacturer: | Vector Laboratories, Inc (ABA) |
| Type: | Recombinant Monoclonal |
| Alias: | Fibroblast growth factor receptor 1; FGFR-1; Basic fibroblast growth factor receptor 1; BFGFR; bFGF-R-1; Fms-like tyrosine kinase 2; FLT-2; N-sam; Proto-oncogene c-Fgr; CD331 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 1 mg |
| Host name: | Rat |
| Clone: | M19B2 |
| Isotype: | IgG1 |
| Immunogen: | Balb/c mice were immunized with recombinant human FGF-R1a or with both recombinant human FGF-R1b and recombinant human FGF-R1a . |
| Application: | FC, IF, IP, WB, RIA, IHC |
Additional Text
Gene Name
FGFR1
Gene ID
2260
Uniprot ID
P11362
Purification
Purified
Antibody Clonality
Recombinant Monoclonal
Storage Note
Store at 4⁰C for up to 3 months. Note, this antibody is provided without added preservatives, it is therefore recommed this antibody be handled under sterile conditions. For longer storage, aliquot and store at -20⁰C.
Application Notes
To characterize this antibody a western blot was preformed on sf9 cells transfected with baculovirus recombinantly expressing the full length FGF-R1 protein. Furthermore immunoprecipitation was preformed on the lysed sf9 cells transfected with baculovirus recombinantly expressing the full length FGF-R1 protein. And finally a radioimmunityassay was preformed with the mouse version of this antibody on I125-labeled complexes (Xu et al, 1992; pmid:1325449). During research on the localization and behaviour of the FGF-1 protein the murine version of this antibody was used to immunoprecipitate FGFR and a 40-kDa complex it is part of (Feng et al, 1995; pmid:9244177). To confirm that Klotho-expressing B cells could respond to FGF23, their expression of FGFR1 was assayed by flow cytometry and immunohistochemical staining. For this the mouse version of this antibody was used (Nakashima et al, 2016; pmid:28095739). fluorescence immunocytochemistry was preformed on brain slices or brain cells from FGF-2 mice and FGF-2 knock-out mice using the mouse version of this antibody. This was done to prove that nuclear FGFR1 and Nurr1 are expressed in the nuclei of developing TH-positive cells in the embryonic ventral midbrain (Baron et al, 2012; pmid:22514272). While investigating a method to establish functional and stable neovessel networks to increase plaque stability The murine version of this antibody was used for western blot on proteins extracted from rabbit aortas and cell lysates (Mao et al, 2019; pmid:31755222).
Short Description
This chimeric rat antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
AB02531-6.1-BT Datasheet
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