Anti-Sodium-dependent serotonin transporter [1-4A5]

Catalogue Number: AB02547-10.0-ABA

Manufacturer:Vector Laboratories, Inc (ABA)
Type:Recombinant Monoclonal
Alias:SLC6A4; SERT; 5HT transporter; 5HTT; Solute carrier family 6 member 4
Shipping Condition:Blue Ice
Unit(s): 200 ug
Host name: Human
Clone: 1-4A5
Isotype: IgG1
Immunogen: This antigen was raised by immunizing a BALB/c mouse with the sodium-dependent serotonin transporter antigen.
Application: ICC, IF, WB, IHC

Additional Text

Gene Name

SLC6A4

Uniprot ID

P31645

Gene ID

6532

Purification

Purified

Antibody Clonality

Recombinant Monoclonal

Storage Note

Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.

Application Notes

This antibody was used for immunohistochemistry on monkey midbrain and hypothalamic sections (Lu et al, 2003; pmid:12660809). Immunofluorescence was preformed on rat neurons using this antibody (Wersinger and Sidhu, 2009; pmid:19429025). SERT protein was analyzed with a western blot using this antibody (Chamba et al, 2008; pmid:18783838). Immunocytochemistry was preformed on stable Bcl-2 transfectants of EBV-ve L3055 Burkitt's lymphoma cells with this antibody (Chamba et al, 2008; pmid:18783838). This antibody was used for immunohistochemistry on mouse brain sections (Zhu et al, 2011; pmid:21705486). Pellets from the centrifuged homogenized rhesus monkey midbrain pontine blocks containing membrane-bound proteins were immonublotted with this antibody (Smith et al, 2004; pmid:15199371). Western blot was preformed on a human colon adenocarcinoma Caco-2 cell line using this antibody (Mastrangelo et al, 2008; pmid:18593952).

Short Description

This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.