Anti-Alpha-ketoglutarate-dependent dioxygenase FTO [5-2H10]

Catalogue Number: AB02550-10.0-BT-ABA

Manufacturer:	Vector Laboratories, Inc (ABA)
Type:	Recombinant Monoclonal
Shipping Condition:	Blue Ice
Unit(s):	1 mg
Host name:	Human
Clone:	5-2H10
Isotype:	IgG1
Immunogen:	This antigen was raised by immunizing a BALB/c mouse with the alpha-ketoglutarate-dependent dioxygenase FTO antigen.
Application:	ICC, WB

Overview
Datasheets

Additional Text

Gene ID

79068

Gene Name

FTO

Uniprot ID

Q9C0B1

Purification

Purified

Antibody Clonality

Recombinant Monoclonal

Specificity

FTO; Fat mass and obesity-associated protein; U6 small nuclear RNA (2'-O-methyladenosine-N(6)-)-demethylase FTO; U6 small nuclear RNA N(6)-methyladenosine-demethylase FTO; mRNA (2'-O-methyladenosine-N(6)-)-demethylase FTO; m6A(m)-demethylase FTO; mRNA N(6)-methyladenosine demethylase FTO; tRNA N1-methyl adenine demethylase FTO

Storage Note

Store at 4⁰C for up to 3 months. Note, this antibody is provided without added preservatives, it is therefore recommed this antibody be handled under sterile conditions. For longer storage, aliquot and store at -20⁰C.

Application Notes

This antibody was used to assess the expression of the FTO protein in cell line 3T3-L1. The method used was a western blot (Jiao et al, 2016; pmid:26907332). Immunocytochemistry with this antibody was used for examination of FTO expression in HOC313, MDA-MB-231, SAS, HeLa, 293FT, and MKN45 cells. The experiment was preformed on normal cells and FTO knock-out cells (Hirayama et al, 2020; pmid:32268083).

Short Description

This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG2a format, for improved compatibility with existing reagents, assays and techniques.

AB02550-10.0-BT Datasheet

Price

£1,655.00

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