Anti-CTLA4 [1B8]
Catalogue Number: AB02559-22.0-ABA
| Manufacturer: | Vector Laboratories, Inc (ABA) |
| Type: | Recombinant Monoclonal |
| Alias: | Cytotoxic T-lymphocyte protein 4; Ctla4; Cytotoxic T-lymphocyte-associated antigen 4; CTLA-4; CD152; UC10-4FIO |
| Shipping Condition: | Blue Ice |
| Unit(s): | 200 ug |
| Host name: | Armenian Hamster |
| Clone: | 1B8 |
| Isotype: | IgG |
| Immunogen: | The antibody was generated by immunizing Armenian hamsters with the extracellular portion of murine CTLA-4 fused to a murine IgG2. |
| Application: | ELISA, FC, IF, WB, Blk |
Additional Text
Gene ID
12477
Gene Name
CTLA4
Uniprot ID
P09793
Purification
Purified
Antibody Clonality
Recombinant Monoclonal
Storage Note
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Application Notes
ELISA was preformed on murine CTLA-4lg to test the binding specificity of the Antibody (Walunas et al, 1994; pmid:7882171). For flow cytometry on A20, M12.41 (B cells), L cell (fibroblast), and P815 (mastocytoma) cells this antibody was used (Perkins et al, 1996; pmid:8666782). This antibody was used for blocking in mice. It prevented T-cell proliferation (Leach et al, 1996; pmid:8596936). Western blot was preformed on LN cells harvested from SJL mice using this antibody (Aruna et al, 2005; pmid:16014414). This antibody was used for flow cytometry on LN cells harvested from SJL mice (Aruna et al, 2005; pmid:16014414). Mouse CD4+ CD25+ T cells were used for a flow cytomic experiment using this antibody (Valsazina et al, 2006; pmid:16618776). Thymi from Tg4 CTLA-4WT and B10.PL mice were immunostained with this antibody (Verhagen et al, 2012; pmid:23267099). LN cells from mice were immunostained with this antibody (Paas-Rozner et al, 2003; pmid:12743364). Cultured CD4+CD25+ Tregs of mice were used in an flow cytometry experiment in which this antibody was used (Wang et al, 2010; pmid:20843956).
AB02559-22.0 Datasheet
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