Anti-estrogen receptor alpha [D75]

Catalogue Number: AB02565-10.3-ABA

Manufacturer:	Vector Laboratories, Inc (ABA)
Type:	Recombinant Monoclonal
Alias:	Estrogen receptor; ER; ER-alpha; Estradiol receptor; Nuclear receptor subfamily 3 group A member 1; ESR1
Shipping Condition:	Blue Ice
Unit(s):	200 ug
Host name:	Human
Clone:	D75
Isotype:	IgG1
Immunogen:	This antibody was raised by immunizing a Lewis rat, with purified estradiol-receptor complex of calf uterine nuclei.
Application:	ELISA, IF, IP, WB, IHC, FA, EMSA

Overview
Datasheets

Additional Text

Gene ID

2099

Gene Name

ESR1

Uniprot ID

P03372

Purification

Purified

Antibody Clonality

Recombinant Monoclonal

Storage Note

Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.

Application Notes

Crude nuclear E*R of calf uterus (0.1 pmol), normal rat serum (10 Ml), and hybridoma medium were used in a double antibody immunoprecipitation with this antibody (Greene et al, 1980; pmid:6928610). ELISA was preformed with this antibody on human MCF-7 cell cytosol (Greene et al, 1982; pmid:6979662). Fresh breast cancer tumor was immunostained using this antibody (Miller et al, 1993; pmid:8490902). Sucrose Density Gradient Analysis was preformed on an extract made from MCF-7 human breast cancer cell line using this antibody (Wong et al, 1991; pmid:1705046). This antibody was used for immunohistochemistry on human breeast cancer (Wong et al, 1991; pmid:1705046). ELISA was preformed on human tumor whole-cell extract using this antibody (Scott et al, 1991; pmid:1864980). An immunohistochemistry was preformed on human breast cancer tissue using this antibody (Miller et al, 1993; pmid:8490902). Immunohistochemistry was preformed on tumor cells from humans with recurrent breast cancer. For this experiment this antibody was used (Rasmussen and Kamby, 1989; pmid:2616369). Western blot was preformed on SV40-transformed HBEC, and MCF-7, T47D, MDA-MB-231 cells using this antibody (Kang et al, 1997; pmid:9054615). Extracts from infected Sf9 cells were analyzed by Western blotting using this antibody (Beekman et al, 1993; pmid:8264659). Gel shift was preformed on human breast tumor sample and control MCF-7 extracts using this antibody (Montgomery et al, 1993; pmid:8219255).

Short Description

This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.

AB02565-10.3 Datasheet

Price

£390.00

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