Anti-estrogen receptor alpha [D547]

Catalogue Number: AB02566-23.0-ABA

Manufacturer:	Vector Laboratories, Inc (ABA)
Type:	Recombinant Monoclonal
Alias:	Estrogen receptor; ER; ER-alpha; Estradiol receptor; Nuclear receptor subfamily 3 group A member 1; ESR1
Shipping Condition:	Blue Ice
Unit(s):	200 ug
Host name:	Rabbit
Clone:	D547
Isotype:	IgG
Immunogen:	This antibody was raised by immunizing a Lewis rat, with purified estradiol-receptor complex of calf uterine nuclei
Application:	ELISA, IF, IP, IHC, FA, EIA

Overview
Datasheets

Additional Text

Gene ID

2099

Gene Name

ESR1

Uniprot ID

P03372

Purification

Purified

Antibody Clonality

Recombinant Monoclonal

Storage Note

Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.

Application Notes

crude nuclear E*R of calf uterus (0.1 pmol), normal rat serum (10 Ml), and hybridoma medium were used in a double antibody immunoprecipitation with this antibody (Greene et al, 1980; pmid:6928610). ELISA was preformed with this antibody on human MCF-7 cell cytosol (Greene et al, 1982; pmid:6979662). Fresh breast cancer tumor was immunostained using this antibody (Miller et al, 1993; pmid:8490902). An EIA was preformed on cytosol from breast cancer tumors using this antibody (Cren et al, 1991; pmid:1911441). COS-7 cells cotransfected with expression plasmids encoding ERa and HA-tagged ERb were immunoprecipitated with this antibody (Ogawa et al, 1998; pmid:9473491). Sucrose Density Gradient Analysis was preformed on GH3 cells (Tate et al, 1984; pmid:6708522). Immunocytochemistry was preformed on the reproductive tract of Female cynomolgus macaques using this antibody (McClellan et al, 1984; pmid:6373239). [3H]E2-labeled macaque E2 receptors were examined on sucrose density gradients using this antibody (McClellan et al, 1984; pmid:6373239).

Short Description

This chimeric rabbit antibody was made using the variable domain sequences of the original rat IgG2a format, for improved compatibility with existing reagents, assays and techniques.

AB02566-23.0 Datasheet

Price

£390.00

-

Quantity
Unit Size

Find out about delivery