Anti-estrogen receptor alpha [D547]
Catalogue Number: AB02566-7.1-BT-ABA
| Manufacturer: | Vector Laboratories, Inc (ABA) |
| Type: | Recombinant Monoclonal |
| Alias: | Estrogen receptor; ER; ER-alpha; Estradiol receptor; Nuclear receptor subfamily 3 group A member 1; ESR1 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 1 mg |
| Host name: | Rat |
| Clone: | D547 |
| Isotype: | IgG2a |
| Immunogen: | This antibody was raised by immunizing a Lewis rat, with purified estradiol-receptor complex of calf uterine nuclei |
| Application: | ELISA, IF, IP, IHC, FA, EIA |
Additional Text
Gene ID
2099
Gene Name
ESR1
Uniprot ID
P03372
Purification
Purified
Antibody Clonality
Recombinant Monoclonal
Storage Note
Store at 4⁰C for up to 3 months. Note, this antibody is provided without added preservatives, it is therefore recommed this antibody be handled under sterile conditions. For longer storage, aliquot and store at -20⁰C.
Application Notes
crude nuclear E*R of calf uterus (0.1 pmol), normal rat serum (10 Ml), and hybridoma medium were used in a double antibody immunoprecipitation with this antibody (Greene et al, 1980; pmid:6928610). ELISA was preformed with this antibody on human MCF-7 cell cytosol (Greene et al, 1982; pmid:6979662). Fresh breast cancer tumor was immunostained using this antibody (Miller et al, 1993; pmid:8490902). An EIA was preformed on cytosol from breast cancer tumors using this antibody (Cren et al, 1991; pmid:1911441). COS-7 cells cotransfected with expression plasmids encoding ERa and HA-tagged ERb were immunoprecipitated with this antibody (Ogawa et al, 1998; pmid:9473491). Sucrose Density Gradient Analysis was preformed on GH3 cells (Tate et al, 1984; pmid:6708522). Immunocytochemistry was preformed on the reproductive tract of Female cynomolgus macaques using this antibody (McClellan et al, 1984; pmid:6373239). [3H]E2-labeled macaque E2 receptors were examined on sucrose density gradients using this antibody (McClellan et al, 1984; pmid:6373239).
AB02566-7.1-BT Datasheet
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