Anti-H-2Kb MHC [AF6-88.5.5.3]

Catalogue Number: AB02570-10.0-ABA

Manufacturer:	Vector Laboratories, Inc (ABA)
Type:	Recombinant Monoclonal
Alias:	H-2 class I histocompatibility antigen, K-B alpha chain; H-2K(B); H2-K1; H2-K
Shipping Condition:	Blue Ice
Unit(s):	200 ug
Host name:	Human
Clone:	AF6-88.5.5.3
Isotype:	IgG1
Immunogen:	This antibody was produced by the fusion of mouse myeloma Sp2/0 cells with spleen cells from a rat, that was immunized with the H-2 class I histocompatibility antigen.
Application:	FC, IF, IP, Inh

Overview
Datasheets

Additional Text

Gene ID

14972

Purification

Purified

Uniprot ID

P01901

Gene Name

H2-K1

Antibody Clonality

Recombinant Monoclonal

Storage Note

Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.

Application Notes

Cloned mouse Th cells L2, grown on CBA/J stimulating spleen cells were used for flow cytometry using this antibody (Wall et al, 1983; pmid:6193171). The inhibition of 125I-7 1-54.3 binding to mouse spleen cel was tested using this antibody (Wall et al, 1983; pmid:6193171). Cells isolated from the spleen, BM, and peripheral blood of transplanted and control animals were assayed by flow cytometry using this antibody (Bjornson et al, 1999; pmid:9915700). Mouse spleen leukocytes were analyzed in flow cytometry using this antibody (Xie et al, 2006; pmid:17050600). Plenic lymphocytes derived from transgenic mice expressing wild-type (WT) H-2Kb, ΔY or Δ7. H-2Kb were used for immunoprecipitation. In this procedure the proteins were immunoprecipitated using this antibody (Lizee et al, 2003; pmid:14566337). Peripheral blood leukocytes from mice were used in a flow cytometry assay using this antibody (Lizee et al, 2003; pmid:14566337). Immunofluorescence was preformed on mouse mature spleen-derived DCs using this antibody (Lizee et al, 2003; pmid:14566337). M1 cells, a clonal isolate derived from B16-F10 murine melanoma cells, were immunostained using this antibody (Hauser et al, 1993; pmid:8467514).

Short Description

This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG2a format, for improved compatibility with existing reagents, assays and techniques.

AB02570-10.0 Datasheet

Price

£390.00

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