Anti-HSV-1 VP5 [6F10-D2-G2]
Catalogue Number: AB02627-15.0-BT-ABA
| Manufacturer: | Vector Laboratories, Inc (ABA) |
| Type: | Recombinant Monoclonal |
| Alias: | Herpes simplex virus type 1 (HSV-1) capsid protein VP5; UL19; Major capsid protein; MCP |
| Shipping Condition: | Blue Ice |
| Unit(s): | 500 ug |
| Host name: | Human |
| Clone: | 6F10-D2-G2 |
| Isotype: | IgM |
| Immunogen: | The original mouse version of this antibody was raised by immunizing a mouse with purified HSV-1 B capsids and recognizes amino acids 862-880 of VP5, the major capsid protein. |
| Application: | ELISA, IF, IP, WB, FA |
Additional Text
Purification
Purified
Antibody Clonality
Recombinant Monoclonal
Storage Note
Store at 4⁰C for up to 3 months. Note, this antibody is provided without added preservatives, it is therefore recommed this antibody be handled under sterile conditions. For longer storage, aliquot and store at -20⁰C.
UniProt ID
G8HBD2
Application Notes
To research the capsid of HSV-1, the mouse version of this antibody was used to immunoprecipitate the capsid of HSV-1 and characterized using ELISA on HSV-1 capsids (Newcomb et al., 1996; PMID: 8918599). To assess the structure of the herpes simplex virus capsid, proteins from BHK-21 cells infected with HSV-1 were immunoblotted with the mouse version of this antibody. Furthermore, the proteins from the BHK-21 cells infected with HSV-1 were immunoprecipitated with the mouse version of this antibody. The immunoprecipitated capsids bound to the mouse version of this antibody were then inspected using cryoelectron microscopy (Spencer et al., 1997; PMID: 9123829). While characterizing the mechanism of HSV-1 nucleocapsid egress, the mouse version of this antibody was used for immunofluorescence on Vero cells transfected with chimeric EGFP and/or infected with HSV-1 (Guan et al., 2014; PMID: 25036476).
Short Description
This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
AB02627-15.0-BT Datasheet
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