Anti-Mouse Nidogen/enactin [JF3 (4H5-B2-E4)]
Catalogue Number: AB02632-23.0-BT-ABA
| Manufacturer: | Vector Laboratories, Inc (ABA) |
| Type: | Recombinant Monoclonal |
| Alias: | Nidogen-1; Nid1; Entactin; NID-1 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 1 mg |
| Host name: | Rabbit |
| Clone: | JF3 (4H5-B2-E4) |
| Isotype: | IgG |
| Immunogen: | This antibody was raised by immunizing a rat with G1/link region of mouse nidogen that is able to block its binding to laminin. |
| Application: | ELISA, IHC, SPR |
Additional Text
Gene Name
NID1
Uniprot ID
P10493
Gene ID
18073
Purification
Purified
Antibody Clonality
Recombinant Monoclonal
Storage Note
Store at 4⁰C for up to 3 months. Note, this antibody is provided without added preservatives, it is therefore recommed this antibody be handled under sterile conditions. For longer storage, aliquot and store at -20⁰C.
Application Notes
The binding of the mouse version of this antibody to nidogen-1 was proven by ELISA. Further, high binding affinity of the mouse version of this antibody to nidogen-1 was demonstrated using surface plasmon resonance (Ries et al, 2001; pmid:11589703). Frozen sections of fetal, newborn, and 4-week-old mice were stained using the mouse version of this antibody to use for immunohistochemistry. This was performed while researching Collagen XXVIII (Veit et al, 2006; pmid:16330543). Frozen and paraffin-embedded sections of fetal, newborn, 3-week-old, and adult mice were stained using the mouse version of this antibody to use for immunohistochemistry. This assay was preformed while researching a protein called AMACO (Sengle et al, 2003; pmid:14506275). The mouse version of this antibody was used to stain whole mouse embryos or dissected organs from mice for immunohistochemistry. The staining allowed for the immunolocalization of nidogen-1 (Kostka et al, 2001; pmid:11564885).
Short Description
This chimeric rabbit antibody was made using the variable domain sequences of the original rat IgG2a format, for improved compatibility with existing reagents, assays and techniques.
AB02632-23.0-BT Datasheet
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