Anti-Fibronectin [L19 (Radretumab)]

Catalogue Number: AB02644-10.3-ABA

Manufacturer:	Vector Laboratories, Inc (ABA)
Type:	Recombinant Monoclonal
Alias:	FN; Cold-insoluble globulin; CIG; EDB(+) FN; ED-B domain of fibronectin; Extra domain-B containing fibronectin
Shipping Condition:	Blue Ice
Unit(s):	200 ug
Host name:	Human
Clone:	L19 (Radretumab)
Isotype:	IgG1
Immunogen:	The original antibody was generated using phage display from a human antibody library.
Application:	ELISA, IF, RIA, IHC

Overview
Datasheets

Additional Text

Gene Name

FN1

Gene ID

2335

Uniprot ID

P02751

Purification

Purified

Antibody Clonality

Recombinant Monoclonal

Storage Note

Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.

Short Description

This full-length, reformatted human antibody was made using the variable domain sequences of the original Human scFv format, for improved compatibility with existing reagents, assays and techniques.

Application Notes

The original version of this antibody (human scFv) was determined to bind human fibronectin at the ED-B domain with a Kd of 54 pM using SPR. Furthermore, the antibody forms a stable complex with the fluorescently labeled ED-B and exhibits a long half-life when complexed with it (Pini et al., 1998; PMID: 9705314). This antibody was used for ELISA on biotinylated ED-B. Furthermore, it was used for a radioimmunoassay conducted on mice with subcutaneously implanted murine F9 teratocarcinoma, and for immunohistochemistry and Immunofluorescence performed on the corneas of New Zealand white rabbits (US8097254). Various formats of this antibody were used in an ELISA on the ED-B domain of FN (Borsi et al, 2002; PMID: 12353237). This antibody was used for immunohistochemistry on tissues from patients with HNSCC (Birchler et al., 2003; PMID: 12838025). A radiolabeled version of this antibody was used for SPECT/CT on patients with SCC (Birchler et al., 2007; PMID: 17418248).

AB02644-10.3 Datasheet

Price

£390.00

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