Anti-14G2a [4B5]

Catalogue Number: AB02667-10.3-ABA

Manufacturer:Vector Laboratories, Inc (ABA)
Type:Recombinant Monoclonal
Alias:antibody 14G2a; ganglioside; ganglioside 2a
Shipping Condition:Blue Ice
Unit(s): 200 ug
Host name: Human
Clone: 4B5
Isotype: IgG1
Immunogen: The original human version of this antibody was generated by treating humans with the 14G2a antibody then MNCs were harvested and hybridized with the murine myeloma cell line P3X.Ag8.653.
Application: ELISA, RIA, FA

Additional Text

Purification

Purified

Antibody Clonality

Recombinant Monoclonal

Storage Note

Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.

Application Notes

To determine the functionality of the mouse version of this antibody, an ELISA was performed to test if this antibody was able to bind to antigen 14G2A. Furthermore, the antibody was also tested in a western blot on antigen 14G2A (EP1000082A2). A radioimmunoassay was performed to screen for anti-14G2a activity of the human version of this antibody. To assess the ability of the human version of this antibody to mediate a cellular immune response. Rabbits were immunized with 4B5. Then, irradiated Mel-21 cells were implanted. The result was induration at the site of the irradiated cells (Saleh et al., 1993; PMID: 8376782).