Anti-14G2a [4B5]
Catalogue Number: AB02667-10.3-BT-ABA
| Manufacturer: | Vector Laboratories, Inc (ABA) |
| Type: | Recombinant Monoclonal |
| Alias: | antibody 14G2a; ganglioside; ganglioside 2a |
| Shipping Condition: | Blue Ice |
| Unit(s): | 1 mg |
| Host name: | Human |
| Clone: | 4B5 |
| Isotype: | IgG1 |
| Immunogen: | The original human version of this antibody was generated by treating humans with the 14G2a antibody then MNCs were harvested and hybridized with the murine myeloma cell line P3X.Ag8.653. |
| Application: | ELISA, RIA, FA |
Additional Text
Purification
Purified
Antibody Clonality
Recombinant Monoclonal
Storage Note
Store at 4⁰C for up to 3 months. Note, this antibody is provided without added preservatives, it is therefore recommed this antibody be handled under sterile conditions. For longer storage, aliquot and store at -20⁰C.
Application Notes
To determine the functionality of the mouse version of this antibody, an ELISA was performed to test if this antibody was able to bind to antigen 14G2A. Furthermore, the antibody was also tested in a western blot on antigen 14G2A (EP1000082A2). A radioimmunoassay was performed to screen for anti-14G2a activity of the human version of this antibody. To assess the ability of the human version of this antibody to mediate a cellular immune response. Rabbits were immunized with 4B5. Then, irradiated Mel-21 cells were implanted. The result was induration at the site of the irradiated cells (Saleh et al., 1993; PMID: 8376782).
AB02667-10.3-BT Datasheet
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