Anti-Mouse Nidogen/enactin [JF4 (5G12-D4-E3)]

Catalogue Number: AB02676-23.0-ABA

Manufacturer:	Vector Laboratories, Inc (ABA)
Type:	Recombinant Monoclonal
Alias:	Nidogen-1; Nid1; Entactin; NID-1
Shipping Condition:	Blue Ice
Unit(s):	200 ug
Host name:	Rabbit
Clone:	JF4 (5G12-D4-E3)
Isotype:	IgG
Immunogen:	This antibody was raised by immunizing a rat with he G2 region of mouse nidogen that is able to block its binding to laminin.
Application:	ELISA, FC, IF, WB, IHC, FA, SPR

Overview
Datasheets

Additional Text

Gene Name

NID1

Uniprot ID

P10493

Gene ID

18073

Purification

Purified

Antibody Clonality

Recombinant Monoclonal

Storage Note

Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.

Application Notes

The binding of the mouse version of this antibody to nidogen-1 was proven by ELISA. Further, high binding affinity of the mouse version of this antibody to nidogen-1 was demonstrated using surface plasmon resonance (Ries et al, 2001; pmid:11589703). While researching nidogen in healthy human cartilage and in late-stage osteoarthritis cartilage an inhibition assay was preformed using the mouse version of this antibody. In short, wells were coated with either nidogen-1 or nidogen-2 to which this antibody was added. Then, chondrocytes were added and their adhesion to the nidogen was tested. 30-40% of chondrocytes showed adhesion to nidogen-1. (Kruegel et al, 2008; pmid:18438862). Tissue sections from Female New Mexico Research Institute (NMRI) mice were stained for immunohistochemistry using this antibody as a positive control. This experiment was preformed to assess the binding between laminin and nidogen (Gersdorff et al, 2005; pmid:15824114). Tissue sections from aborted human embryos were stained with the mouse version of this antibody to be used for immunohistochemistry (Miosge et al, 2001; pmid:12005023). In research on osteoarthritis multiple experiments were preformed using the mouse version of this antibody. First, tissues from DDR-1 KO mice were stained for immunohistochemistry using the mouse version of this antibody. Second, immunoblotting was preformed on proteins extracted from DDR-1 KO mice. Third, this antibody was used to stain cell cultures taken from TMJ cartilage tissue of 9-week-old DDR-1 KO mice and their WT littermates. Finally, the cell cultures taken from TMJ cartilage tissue of 9-week-old DDR-1 KO mice and their WT littermates were used for flow cytometry and stained with this antibody (Schminke et al, 2014; pmid:23912900). In research on nidogen-2, the mouse version of this antibody was used to immunolocalize nidogen-1 in both immunofluorescence and immunohistochemistry on cells and tissues from mice with a mutated nidogen-2 gene (Schymeinsky et al, 2002; pmid:12215539).

Short Description

This chimeric rabbit antibody was made using the variable domain sequences of the original Rat IgG2b format, for improved compatibility with existing reagents, assays and techniques.

AB02676-23.0 Datasheet

Price

£390.00

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