Anti-hemagglutinin H1 [5589]
Catalogue Number: AB02714-10.0-ABA
| Manufacturer: | Vector Laboratories, Inc (ABA) |
| Type: | Recombinant Monoclonal |
| Alias: | H1; haemagglutinin H1; H1-specific; #5889 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 200 ug |
| Host name: | Human |
| Clone: | 5589 |
| Isotype: | IgG1 |
| Immunogen: | This antibody was raised by sequencing DNA from PMBCs from humans immunized with the split-virion (IIV) quadrivalent seasonal influenza vaccine Fluzone in 2016-2017. |
| Application: | ELISA, FC, FA |
Additional Text
Purification
Purified
Antibody Clonality
Recombinant Monoclonal
Storage Note
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Application Notes
ELISA on recombinant hemagglutinnin using the human version of this antibody has proven the specificity of this antibody. Further, hemagglutination inhibition assays (HAIs) were also performed to measure functional antibodies capable of inhibiting virus agglutination. For this assay the human antibody was mixed with the influenza virus to which erythrocytes were added. Then the HAI titer was determined by the reciprocal dilution of the last well that contained non-agglutinated RBCs. Finally, human PBMCs were stained using the human version of this antibody to be used for flow cytometry (Forgacs et al, 2021; pmid:33617543).
AB02714-10.0 Datasheet
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