Catalogue Number: AB02714-10.0-BT-ABA
| Manufacturer: | Vector Laboratories, Inc (ABA) |
| Type: | Recombinant Monoclonal |
| Alias: | H1; haemagglutinin H1; H1-specific; #5889 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 1 mg |
| Host name: | Human |
| Clone: | 5589 |
| Isotype: | IgG1 |
| Immunogen: | This antibody was raised by sequencing DNA from PMBCs from humans immunized with the split-virion (IIV) quadrivalent seasonal influenza vaccine Fluzone in 2016-2017. |
| Application: | ELISA, FC, FA |
Purified
Recombinant Monoclonal
Store at 4⁰C for up to 3 months. Note, this antibody is provided without added preservatives, it is therefore recommed this antibody be handled under sterile conditions. For longer storage, aliquot and store at -20⁰C.
ELISA on recombinant hemagglutinnin using the human version of this antibody has proven the specificity of this antibody. Further, hemagglutination inhibition assays (HAIs) were also performed to measure functional antibodies capable of inhibiting virus agglutination. For this assay the human antibody was mixed with the influenza virus to which erythrocytes were added. Then the HAI titer was determined by the reciprocal dilution of the last well that contained non-agglutinated RBCs. Finally, human PBMCs were stained using the human version of this antibody to be used for flow cytometry (Forgacs et al, 2021; pmid:33617543).