Anti-hemagglutinin H1 [3978]

Catalogue Number: AB02717-10.3-ABA

Manufacturer:Vector Laboratories, Inc (ABA)
Type:Recombinant Monoclonal
Alias:H1; haemagglutinin H1; #3978
Shipping Condition:Blue Ice
Unit(s): 200 ug
Host name: Human
Clone: 3978
Isotype: IgG1
Immunogen: This antibody was raised by sequencing DNA from PMBCs from humans immunized with the split-virion (IIV) quadrivalent seasonal influenza vaccine Fluzone in 2016-2017.
Application: ELISA, FC, FA

Additional Text

Purification

Purified

Antibody Clonality

Recombinant Monoclonal

Storage Note

Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.

Application Notes

ELISA on recombinant hemagglutinnin has proven the specificity of the human version of this antibody. Further, hemagglutination inhibition assays (HAIs) were also performed to measure functional antibodies capable of inhibiting virus agglutination. For this assay the human antibody was mixed with the influenza virus to which erythrocytes were added. Then, the HAI titer was determined by the reciprocal dilution of the last well that contained non-agglutinated RBCs. Finally, human PBMCs were stained for flow cytometry using the human version of this antibody (Forgacs et al, 2021; pmid:33617543).