Anti-IGF1R [10H5]
Catalogue Number: AB02740-10.0-BT-ABA
| Manufacturer: | Vector Laboratories, Inc (ABA) |
| Type: | Monoclonal Secondary Antibody - Unconjugated |
| Alias: | CD221; IGF1 receptor; Insulin-like growth factor 1 receptor; type I IGF receptor; Insulin-like growth factor I receptor; IGF-I receptor |
| Shipping Condition: | Blue Ice |
| Unit(s): | 1 mg |
| Host name: | Human |
| Clone: | 10H5 |
| Isotype: | IgG1 |
| Immunogen: | The original antibody was generated from BALB/c mice hyperimmunized with human IGF-IR extracellular domain (amino acids 1-902) in Ribi adjuvant. Later on the humanized version was created by grafting the hypervariable regions onto human framework regions. |
| Application: | ELISA, FC, IP |
Additional Text
Gene Name
IGF1R
Gene ID
3480
Uniprot ID
P08069
Purification
Purified
Antibody Clonality
Recombinant Monoclonal
Storage Note
Store at 4⁰C for up to 3 months. Note, this antibody is provided without added preservatives, it is therefore recommed this antibody be handled under sterile conditions. For longer storage, aliquot and store at -20⁰C.
Application Notes
The orginal humanized IgG1 version of this antibody binds human IGF-1R with an affinity of 1.1 nM in an ELISA. The binding specificity was also comfirmed using fluorescence activated cell sorting of IGF-IR-expressing MCF7 cells. This antibody was also used for the immunoprecipitaion of MCF7 cells. The original antibody inhibits IGF-IR-mediated signaling by blocking IGF-I and IGF-II binding and by inducing cell surface receptor downregulation via internalization and degradation, with the extracellular and intracellular domains of IGF-IR being differentially affected by the proteasomal and lysosomal inhibitors. In vitro, h10H5 exhibits antiproliferative effects on cancer cell lines. In vivo, h10H5 shows single-agent antitumor efficacy in human SK-N-AS neuroblastoma and SW527 breast cancer xenograft models and even greater efficacy in combination with the chemotherapeutic agent docetaxel or an anti-vascular endothelial growth factor antibody (PMID:18790743).
AB02740-10.0-BT Datasheet
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