Anti-Spike protein [4A8]

Catalogue Number: AB02743-10.3-ABA

Manufacturer:Vector Laboratories, Inc (ABA)
Type:Recombinant Monoclonal
Shipping Condition:Blue Ice
Unit(s): 200 ug
Host name: Human
Clone: 4A8
Isotype: IgG1
Immunogen: This antibody was isolated from the blood of recovered SARS CoV2 patients.
Application: ELISA, FC, FA, NT

Additional Text

Gene Name

S

Purification

Purified

Antibody Clonality

Recombinant Monoclonal

Gene ID

43740568

Uniprot ID

P0DTC2

Storage Note

Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.

Application Notes

The binding profile of this antibody was determined by performing an ELISA on SARS-CoV-2 S, S1, S2 or RBD protein using the human version of this antibody. Furthermore a neutralization experiment on Vero-E6 cells infected with SARS CoV2 was done using the human IgG1 version of this antibody. The binding of the human version was also characterized using biolayer interferometry. A flow cytometric receptor binding inhibition assay was also performed on the human version of this antibody incubated in SARS-CoV-2S protein and then incubated with ACE2-293T cells. The complex of S-ECD and the human version of this antibody was characterized using cryo electromicroscopy (Chi et al, 2020; pmid:32571838).

Specificity

SARS CoV 2 S glycoprotein; COVID-19 Spike protein; E2 glycoprotein; E2; Human coronavirus 2 spike glycoprotein; Peplomer protein; S glycoprotein; SARS coronavirus 2 S protein; SARS coronavirus 2 Spike Protein; SARS CoV 2 Spike protein; SARS CoV 2; SARS-CoV-2 S protein; SARSCoV2; SARS-COV-2 S protein; SARS-COV-2 Spike glycoprotein; SARSCOV2 Spike protein; Severe acute respiratory syndrome 2 spike glycoprotein; Severe acute respiratory syndrome virus 2 spike glycoprotein; Spike glycoprotein; 2019-nCoV