Anti-Estradiol-17β [E4-4]

Catalogue Number: AB02762-10.0-ABA

Manufacturer:Vector Laboratories, Inc (ABA)
Type:Recombinant Monoclonal
Alias:17-beta-estradiol; E2; ethynyl estradiol; estradiol; oestradiol; ChEBI: 16469
Shipping Condition:Blue Ice
Unit(s): 200 ug
Host name: Human
Clone: E4-4
Isotype: IgG1
Immunogen: This antibody was raised by immunizing a female balb/c mouse with immunogenic Estradiol-17β-BSA conjugate.
Application: ELISA, RIA, SPR

Additional Text

Purification

Purified

Antibody Clonality

Recombinant Monoclonal

Storage Note

Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.

Application Notes

The scFv version of this antibody is capable of binding E2-7-BSA in an ELISA. A radioimmunoassay was performed using E2 standard and E2 with [H3] using the mouse version and the scFv version of this antibody. Finally the association and dissociation rate were determined using surface plasmon resonance on E2-7-BSA using both the mouse version and the scFv version of this antibody (Kobayashi et al, 2008; pmid:18824188) (Kobayashi et al, 2010; pmid:20047279).

Short Description

This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.