Anti-Glycoprotein E2 [AP33]

Catalogue Number: AB02794-23.0-BT-ABA

Manufacturer:	Vector Laboratories, Inc (ABA)
Type:	Recombinant Monoclonal
Alias:	NS1; gp68; gp70; HCV glycoprotein E2; Genome polyprotein; Envelope glycoprotein E2; E2 glycoprotein
Shipping Condition:	Blue Ice
Unit(s):	1 mg
Host name:	Rabbit
Clone:	AP33
Isotype:	IgG
Immunogen:	The original antibody was generated by immunizing a mouse with HCV envelope glycoprotein E2.
Application:	ELISA, IP, FA, NT, Blk

Overview
Datasheets

Additional Text

Purification

Purified

Uniprot ID

P27958

Antibody Clonality

Recombinant Monoclonal

Storage Note

Store at 4⁰C for up to 3 months. Note, this antibody is provided without added preservatives, it is therefore recommed this antibody be handled under sterile conditions. For longer storage, aliquot and store at -20⁰C.

Application Notes

The binding of AP33 inhibits the interaction between CD81 and a range of presentations of E2, including soluble E2, E1E2, and virus-like particles. To asses and the reactivity and neutralizing capacity of antisera and monoclonal antibodies, proteins from HEK293T cells cotransfected with a plasmid containing HCV genotype 1a strain H77-derived full-length E1E2 were immunoprecipitated using the mouse version of this antibody. Further on the same HEK293T cells an ELISA was performed using the mouse version of this antibody. Finally a neutralization assay was performed by incubating huh-7 cells with HCVpp in the presence of the mouse version of this antibody and then measuring the fluorescence using flow cytometry (Owsianka et al, 2005; pmid:16103160). To measure the relative binding affinity of the mouse version of this antibody to HCV E1E2 glycoproteins, a cell lysate-based ELISA was performed. Further HCV pseudo particles (HCVpp) and cell-culture-derived HCV (HCVcc) neutralization assays were performed (Desombere et al, 2016; pmid:26710081). To determine the reactivity of selected phage with the selecting antibody, a phage capture enzyme immunoassay was performed. For this assay the mouse version of this antibody was used. Further to detect MAb binding to E2 glycoprotein, an ELISA was performed on transfected HEK293FT cells using the mouse version of this antibody (Tarr et al, 2006; pmid:16496330). Binding of the mouse version of this antibody was assessed in the presence of the peptide-reactive serum, and HCV-positive, peptide-unreactive serum and normal human serum. Further to determine the neutralization ability of this antibody a neutralization assay was performed on huh-7 cells and HEK293T cells infected with HCVpps (Tarr et al, 2007; pmid:17947521).

Short Description

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format for improved compatibility with existing reagents assays and techniques.

AB02794-23.0-BT Datasheet

Price

£1,655.00

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