Anti-PD-1 [29F.1A12]
Catalogue Number: AB02816-7.4-BT-ABA
| Manufacturer: | Vector Laboratories, Inc (ABA) |
| Type: | Recombinant Monoclonal |
| Alias: | CD279; Programmed cell death protein 1; Protein PD-1; mPD-1 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 1 mg |
| Host name: | Rat |
| Clone: | 29F.1A12 |
| Isotype: | IgG2a |
| Immunogen: | The antibody was raised by immunizing rats with plasmid DNA containing PD-1. |
| Application: | FC, WB, IHC, Blk |
Additional Text
Gene Name
PDCD1
Uniprot ID
Q02242
Gene ID
18566
Purification
Purified
Antibody Clonality
Recombinant Monoclonal
Storage Note
Store at 4⁰C for up to 3 months. Note, this antibody is provided without added preservatives, it is therefore recommed this antibody be handled under sterile conditions. For longer storage, aliquot and store at -20⁰C.
Application Notes
This antibody was used for detection of PD-1 in CHO cells using flow cytometry. The ability of the antibody to block PD-1 interaction with PD-L1 or PD-L2 on CHO cells in an in vitro blocking assay was tested; the clone blocked PD-L1 Ig and PD-L2 Ig binding at high concentration, but only blocked 35% of PD-L1 Ig or PD-L2 Ig binding at 10 ug/ml. This antibody was also used for immunohistochemical studies of various mouse tissues, such as heart (Liang et al, 2003; pmid: 14515254). In vivo anti-tumor effect in terms of level of tumor growth reduction was studied, a significant tumor growth reduction was observed in the tumors from mice treated with the antibody (Tobias et al, 2020; pmid: 32528470). The antibody was used for the detection of PD-1 in EL4 cells using flow cytometry. The antibody was used for cross-blocking with the unconjugated version of the same clones and it was reported to prevent PD-1 from interacting with PD-L1 (Polesso et al, 2021; pmid: 33684223). The antibody was used as a positive control for CT26 tumor model, a murine model of colon cancer (Kaumaya ey al.; pmid:33117602).
AB02816-7.4-BT Datasheet
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