Anti-CEA [T84.66]
Catalogue Number: AB02833-10.3-ABA
| Manufacturer: | Vector Laboratories, Inc (ABA) |
| Type: | Recombinant Monoclonal |
| Alias: | Carcinoembryonic antigen; Carcinoembryonic antigen-related cell adhesion molecule 5; CEACAM5; Meconium antigen 100; CD66e |
| Shipping Condition: | Blue Ice |
| Unit(s): | 200 ug |
| Host name: | Human |
| Clone: | T84.66 |
| Isotype: | IgG1 |
| Immunogen: | The original antibody was generated by immunizing female BALB/c mice subcutaneously with soluble CEA or with the HC84S cell line. |
| Application: | ELISA, FC, RIA, IHC |
Additional Text
Gene Name
CEACAM5
Gene ID
1048
Uniprot ID
P06731
Purification
Purified
Antibody Clonality
Recombinant Monoclonal
Storage Note
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Application Notes
The binding affinity of the original mouse antibody to carcinoembryonic antigen was determined using a competitive radioimmunoassay (PMID: 6187848). 111In (Indacea) labelled version of this antibody was used to image tumors in patients with colorectal cancer (PMID: 3779658). An engineered antibody fragment (VL-VH-CH3, or "minibody") generated using this antibody having bivalent binding to carcinoembryonic antigen showed high level targeting of xenografts (PMID: 8674062). A radiolabeled chimeric version of this antibody was used to target breast cancer cells expressing high levels of CEA and it was found that chimeric 86.66 resulted in good tumor localization as well as significant tumor growth inhibition. This antibody was also used to detect CEA expression in Human breast carcinoma cell line, MCF 7, using flow cytometry and immunohistochemistry (PMID: 21597719). The characterization of the chimeric version of this antibody generated by transient expression in tobacco leaves was done using ELISA (PMID: 10500141). A phase 1 clinical radioimmunotherapy trial studying 22 patients with metastatic CEA-producing malignancies involving 90Yttrium-labeled chimeric T86.44 revealed that the antibody was well tolerated, with reversible thrombocytopenia and leukopenia being dose limiting (PMID: 11051230). This antibody was used in the immunohistochemical staining of paraffin sections of primary tumours from 252 consecutive patients with breast carcinomas (PMID: 9635845).
Short Description
This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format for improved compatibility with existing reagents assays and techniques.
AB02833-10.3 Datasheet
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