Anti-CD99 [12E7]
Catalogue Number: AB02834-10.3-BT-ABA
| Manufacturer: | Vector Laboratories, Inc (ABA) |
| Type: | Recombinant Monoclonal |
| Alias: | CD99 antigen; E2 antigen; Protein MIC2; T-cell surface glycoprotein E2; 12E7; MIC2; MIC2X; MIC2Y |
| Shipping Condition: | Blue Ice |
| Unit(s): | 1 mg |
| Host name: | Human |
| Clone: | 12E7 |
| Isotype: | IgG1 |
| Immunogen: | The original antibody was generated by immunizing BALB/c mice intraperitoneally with glutaraldehyde-fixed human acute lymphocytic leukemia (ALL) cells obtained from peripheral blood of a child. |
| Application: | FC, ICC, IF, WB, RIA, IHC |
Additional Text
Gene ID
4267
Gene Name
CD99
Uniprot ID
P14209
Purification
Purified
Antibody Clonality
Recombinant Monoclonal
Storage Note
Store at 4⁰C for up to 3 months. Note, this antibody is provided without added preservatives, it is therefore recommed this antibody be handled under sterile conditions. For longer storage, aliquot and store at -20⁰C.
Application Notes
The original mouse IgG1 version of this antibody was used for the indirect immunofluorescence staining of a fixed bone marrow smear from a patient with T-cell leukemia. The binding specificity of this antibody towards CD99 was tested using radioimmunoassay (PMID: 316541). This antibody was able to identify 28 kDa component present on lymphocytes, red blood cells and platelets in a western blot assay (PMID: 2447875). This antibody was used in the immunocytochemical studies of Ewing's sarcoma and peripheral neuroectodermal tumour (PMID: 8314240). This antibody was also used in the immunohistochemical studies of formalin-fixed, paraffin-embedded lymphoblastic lymphoma (LyL)/acute lymphoblastic leukemia (ALL) samples collected from 24 patients (PMID: 9343323). This antibody was also used in a study relating to the cell surface expression MIC2 antigen on human leukocytes in bone marrow (BM), thymus, and peripheral blood (PB) using multiparameter flow cytometry and cell sorting (PMID: 7506950).
Short Description
This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format for improved compatibility with existing reagents assays and techniques.
AB02834-10.3-BT Datasheet
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