Anti-SEB [4PCL2]
Catalogue Number: AB02923-23.0-ABA
| Manufacturer: | Vector Laboratories, Inc (ABA) |
| Type: | Recombinant Monoclonal |
| Alias: | Staphylococcal enterotoxin B; Enterotoxin type B; entB; 2F6D9G2 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 200 ug |
| Host name: | Rabbit |
| Clone: | 4PCL2 |
| Isotype: | IgG |
| Immunogen: | The original antibody was generated by immunizing eight-week-old female BALB/c mice were immunized subcutaneously with SEB at 3-week intervals. Later on a phage display library was generated from the variable regions of the hybridoma and this clone was isolated by panning against SEB. |
| Application: | ELISA, WB, SPR |
Additional Text
Purification
Purified
Uniprot ID
P01552
Antibody Clonality
Recombinant Monoclonal
Storage Note
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Application Notes
This antibody can be used for the immundection of SEB in a sample. This antibody was derived from the murine parent antibody called 2F6D9G2, after PCR cloning the variable region genes of the hybridoma to generate a phage display library and isolating the specific clone that showed reactivity towards SEB. The binding reactivity of the scFv clone towards SEB was determined using ELISA. The scFv clone binds SEB with a high affinity of 3.16 nM as determined by surface plasmon resonance (SPR). This scFv antibody was also capable of detecting SEB in a western blot assay (PMID: 20952642).
Short Description
This full-length chimeric rabbit antibody was made using the variable domain sequences of the original Mouse scFv format for improved compatibility with existing reagents assays and techniques.
AB02923-23.0 Datasheet
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