Anti-ABCG2 [5D3]
Catalogue Number: AB02927-3.3-BT-ABA
| Manufacturer: | Vector Laboratories, Inc (ABA) |
| Type: | Recombinant Monoclonal |
| Shipping Condition: | Blue Ice |
| Unit(s): | 1 mg |
| Host name: | Mouse |
| Clone: | 5D3 |
| Isotype: | IgG2b |
| Immunogen: | The original antibody was generated by immunizing mice with intact mouse 3T3 fibroblasts expressing the human ABCG2 protein. |
| Application: | FC, IF, IHC |
Additional Text
Gene Name
ABCG2
Uniprot ID
Q9UNQ0
Gene ID
9429
Purification
Purified
Antibody Clonality
Recombinant Monoclonal
Specificity
CD338; CDw338; ABCP; BCRP; BCRP1; MXR; Breast cancer resistance protein; mitoxantrone resistance; Urate exporter; Broad substrate specificity ATP-binding cassette transporter ABCG2; ATP-binding cassette sub-family G member 2; Mitoxantrone resistance-associated protein; Placenta-specific ATP-binding cassette transporter
Storage Note
Store at 4⁰C for up to 3 months. Note, this antibody is provided without added preservatives, it is therefore recommed this antibody be handled under sterile conditions. For longer storage, aliquot and store at -20⁰C.
Application Notes
This antibody is recommended for identification of human ACBG2 in a sample. This antibody was used in the immunostaining of bone marrow cells to establish a direct link between the SP phenotype and ABCG2 expression (PMID: 11533706). The IgG2b version of this antibody was used to identify AML cell lines that express ABCG2 protein using flow cytometry. This antibody inhibits ABCG2 function, presumably by binding to an external epitope and preventing conformational changes in the transporter, and therefore provides a specific inhibitor assay for ABCG2 function (PMID: 12393637). The SP (side population phenotype) analysis of limbal epithelial cells using 5D3 and TPS-A showed that both 5D3 and TPS-A effectively inhibit Hoechst 33342 dye efflux from these cells (PMID: 15135043). Conformational changes in the ABCG2 multidrug transporter result in the modification of its interaction with 5D3 antibody. This antibody therefore cannot be used to identify human ABCG2 in an immunoblot/western blot assay. The binding of this antibody was maximum in the presence of ATP, ADP, or Ko143, whereas the non-hydrolysable ATP analog, adenosine 5'-(beta, gamma-imido)triphosphate (AMP-PNP), and nucleotide trapping by vanadate decreased antibody binding. In membranes expressing the ABCG2-K86M mutant, ATP, ADP, and AMP-PNP decreased, whereas Ko143 increased the binding of this antibody. 5D3 can be used as a sensitive tool to reveal intramolecular changes, reflecting ATP binding, the formation of a catalytic intermediate, or substrate inhibition within the transport cycle of the ABCG2 protein (PMID: 15557326). This antibody was also used in a study involving localization of ABCG2 mitoxantrone resistance-associated protein in normal tissues using immunohistochemistry (PMID: 15990223). This antibody was also used in determining ABCG2 expression using immunofluorescent staining (PMID: 15625123).
AB02927-3.3-BT Datasheet
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