Catalogue Number: AB02930-30.11-BT-ABA
| Manufacturer: | Vector Laboratories, Inc (ABA) |
| Type: | Recombinant Monoclonal |
| Alias: | RAL; F-2 toxin; F-2 mycotoxin; Mycotoxin F2; [6-(10-hydroxy-6-oxo-trans-1-undecenyl)-bresorcylic acid lactone]; CAS NO: 17924-92-4; ChEBI:10106 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 500 ug |
| Host name: | |
| Clone: | QY1.5 |
| Isotype: | |
| Immunogen: | The original antibody was generated using phage display technology by panning an scFv library against zearalenone-ovalbumin conjugate. The scFv library was created by linking the heavy and light chain genes of antizearalenone hybridoma cell line (2G3-6E3-2E2) using DNA linker encoding peptide (Gly4Ser)3 and cloning into a phagemid (pCANTAB5E) to be expressed as a fusion protein on the surfaces of recombinant phages. The original hybridoma (2G3-6E3-2E2) was generated using zearalenone-BSA conjugate as an immunogen. |
| Application: | ELISA |
Purified
Recombinant Monoclonal
Store at 4⁰C for up to 3 months. Note, this antibody is provided without added preservatives, it is therefore recommed this antibody be handled under sterile conditions. For longer storage, aliquot and store at -20⁰C.
The binding specificity of this scFv antibody towards mycotoxin zearalenone was determined using ELISA. The scFv version of this antibody was also able to detect zearalenone in spiked corn extract in a competitive-indirect ELISA (PMID: 8979354).