Catalogue Number: AB02942-23.0-ABA
| Manufacturer: | Vector Laboratories, Inc (ABA) |
| Type: | Recombinant Monoclonal |
| Alias: | Flagellar biosynthesis protein FlhA |
| Shipping Condition: | Blue Ice |
| Unit(s): | 200 ug |
| Host name: | Rabbit |
| Clone: | 1199 |
| Isotype: | IgG |
| Immunogen: | The original antibody was generated by panning against a naturally expressed murine library. |
| Application: | ELISA, DB |
Purified
Recombinant Monoclonal
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
C6XF33
Candidatus Liberibacter asiaticus (CaLas) is the pathogen that causes citrus Huanglongbing (HLB), the most serious disease of citrus. The paper describes the development of single chain variable fragment (scFv) antibodies against surface proteins of 'Ca. Liberibacter asiaticus' as potential diagnostic methods. A primary library of recombinant single chain variable fragment antibody was developed and the library was screened for antibodies that bind to specifically-chosen proteins that are present on the surface of CaLas. In particular, dot blot identified FlhA as a protein which is expressed on the surface of CaLas and ELISA result showed the antibody 1199 binds Flha (A450=0.26) (PMID 26744234).
This full-length chimeric rabbit antibody was made using the variable domain sequences of the original Mouse scFv format for improved compatibility with existing reagents assays and techniques.