Anti-Sulfatide [O4]
Catalogue Number: AB02985-8.1-ABA
| Manufacturer: | Vector Laboratories, Inc (ABA) |
| Type: | Recombinant Monoclonal |
| Alias: | Oligodendrocyte; sulfated galactolipids; 3'-sulfogalactosylceramide; ; 3'-sulfogalactosyl 1-0-alkyl ether 2-acylglycerol; seminolipid; cholesterol; 04 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 200 ug |
| Host name: | Rat |
| Clone: | O4 |
| Isotype: | IgG2b |
| Immunogen: | The original antibody was generated by immunizing BLAB/c mice with white matter from bovine corpus callosum. |
| Application: | ELISA, FC, ICC, IF, DB, IHC |
Additional Text
Purification
Purified
Antibody Clonality
Recombinant Monoclonal
Storage Note
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Application Notes
Indirect immunofluorescence in cultures of early postnatal mouse cerebellum, cerebrum, spinal cord, optic nerve, and retina was used to study the localization of the antigen bound by this antibody. The original IgM version of this antibody has been used in complement-dependent cytotoxic elimination of cerebellar oligodendrocytes in culture (PMID: 6786942). The reactivity of this antibody towards sulphated glycolipids like sulfatide, seminolipid and cholesterol was texted using ELISA and dot-blots (PMID: 2600978). This antibody along with another oligodendrocyte antibody O4 and oligodendroglial precursor cells (OPC) antibody A2B5 can be used for the immunocytochemical study the developmental stages of oligodendrocytes (PMID: 28230075). O4 antibody specific antigens are expressed on early dendrocyte progenitor and O1 antigens are expressed on late oligodendrocyte progenitors (PMID: 6178060). This antibody was used in the flow cytometric characterization of mouse oligodendroglial cell populations for study of mouse demyelinating disease (PMID: 25247590).
Short Description
This chimeric rat antibody was made using the variable domain sequences of the original Mouse IgM format for improved compatibility with existing reagents assays and techniques.
AB02985-8.1 Datasheet
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