Anti-Diethoxyphosphotyrosine [DepY]

Catalogue Number: AB02994-23.0-ABA

Manufacturer:	Vector Laboratories, Inc (ABA)
Type:	Recombinant Monoclonal
Shipping Condition:	Blue Ice
Unit(s):	200 ug
Host name:	Rabbit
Clone:	DepY
Isotype:	IgG
Immunogen:	The diethoxyphosphorylated (OP) peptides cross-linked to four different carrier proteins were used to immunize and boost mice. Mice were immunized with BSA conjugated to 5 peptides diethoxyphosphorylated on tyrosine: PVYSR-OP, LYGLPR-OP, KYAOP, HYRGPA-OP, and EPNVSY-OP. Mice were boosted with OVA conjugated to three peptides diethoxyphosphorylated on tyrosine, RSLYAS-OP, YGGFL-OP, and YPF-OP, and HSA conjugated to PPYRM-OP and QYDVRK-OP. Later on the hybridomas were screened using lysozyme conjugated to RARYEM-OP, GGYR-OP, and KYK-OP.
Application:	ELISA, WB, SPR

Overview
Datasheets

Additional Text

Purification

Purified

Antibody Clonality

Recombinant Monoclonal

Storage Note

Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.

Application Notes

This antibody was characterized in order to determine its binding affinity and binding specificity. The original mouse antibody recognized diethoxyphosphotyrosine independent of the amino acid sequence around the modified tyrosine and independent of the identity of the carrier protein or peptide in an ELISA and western blotting assay. The original mouse IgG2a version of this antibody had an IC50 of 3 x 10-9 M in a competition assay with OP tubulin. Kd values measured by BIAcore and OctetRED96 were 10-8 M for OP-peptides and 1 x 10-12 M for OP-proteins. The limit of detection measured on western blots hybridized with 0.14 µg/mL of depY was 0.025 µg of human albumin conjugated to YGGFL-OP (PMID: 29137457).

Short Description

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2a format for improved compatibility with existing reagents assays and techniques.

AB02994-23.0 Datasheet

Price

£390.00

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