Anti-Monkeypox and Vaccinia A13 protein [11F7]
Catalogue Number: AB02996-23.0-ABA
| Manufacturer: | Vector Laboratories, Inc (ABA) |
| Type: | Recombinant Monoclonal |
| Alias: | VACV; Mpox; monkeypox virus; Protein P8; A13L; vaccinia virus; smallpox virus; VACV virion membrane proteins; Virion membrane protein A13; VACWR132 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 200 ug |
| Host name: | Rabbit |
| Clone: | 11F7 |
| Isotype: | IgG |
| Immunogen: | The original antibody was generated by immunizing BALB/c mice with WR strain of live vaccinia virus (VACV). |
| Application: | ELISA, IF, WB, NT, SPR |
Additional Text
Purification
Purified
Antibody Clonality
Recombinant Monoclonal
Storage Note
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Gene ID
3707530
Gene Name
A13L
UniProt ID
Q76ZQ4
Short Description
This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2a format for improved compatibility with existing reagents assays and techniques.
Application Notes
The original IgG2a version of this antibody bound to recombinant A13 protein expressed in E. coli, MBP conjugated VACV A13 in an ELISA. The original IgG2a antibody bound recombinant A13 proteins from E. coli with an affinity of 3.4 nM as detected by surface plasmon resonance. This antibody was also capable of recognizing a 12-kda protein in a Western blot of VACV-infected HeLa cells. This antibody stained viral factories and virion-size particles in immunofluorescence analysis of infected cells. This antibody was also reported to neutralized VACV mature virions in a plaque reduction assay. Passive immunization of mice with this antibody protected mice against intranasal VACV infection. This antibody generated against smallpox vaccine might contribute to immune protection against orthopoxviruses (PMID: 21810533). In Q3 of 2024, the WHO declared monkeypox a Public Health Emergency of International Concern. This product serves as a powerful tool for related research efforts.
AB02996-23.0 Datasheet
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