Anti-TRKB [C2]
Catalogue Number: AB03000-3.3-BT-ABA
| Manufacturer: | Vector Laboratories, Inc (ABA) |
| Type: | Recombinant Monoclonal |
| Alias: | NTRK2; Trk-B; GP145-TrkB; TrkB tyrosine kinase; Tropomyosin-related kinase; BDNF/NT-3 growth factors receptor; Neurotrophic tyrosine kinase receptor type 2; Tropomyosin receptor kinase B; tyrosine receptor kinase B |
| Shipping Condition: | Blue Ice |
| Unit(s): | 1 mg |
| Host name: | Mouse |
| Clone: | C2 |
| Isotype: | IgG2b |
| Immunogen: | The original antibody was generated by grafting of the murine antibody 38B8 CDRs onto human framework regions. The parental murine antibody 38B8 was generated by immunizing mice with human TRKB. |
| Application: | ELISA, Blk, Agon |
Additional Text
Gene Name
NTRK2
Gene ID
4915
Uniprot ID
Q16620
Purification
Purified
Antibody Clonality
Recombinant Monoclonal
Storage Note
Store at 4⁰C for up to 3 months. Note, this antibody is provided without added preservatives, it is therefore recommed this antibody be handled under sterile conditions. For longer storage, aliquot and store at -20⁰C.
Application Notes
This agonist antibody is derived from the murine parental anti-TRKB antibody 38B8. The binding of this antibody to TRKB blocks binding of BDNF. The original IgG1 version of this antibody has a KD for human TrkB of 2.54 nM, a KD for mouse TrkB of 1.44 nM, and a KD for rat TrkB of 2.29 nM when measured by Biacore at 25° C. The binding affinity of Fab version of this antibody towards human, mouse and rat TRKB was tested using ELISA. This antibody was reported to show greater efficacy in promoting mouse neuron survival than the parental mouse antibody and BDNF. In vivo treatments with this antibody of mice on high fat diet-induced obesity showed changes in body weight. A single intraperitoneal dose of C2 at 2 mg/kg caused a statistically significant body weight reduction compared to vehicle-treated mice as early as day 2 after dosing. The mean maximum weight reduction by C2 antibody was 29.05% by day 14 when the animals were euthanized (US20100196390). This antibody may also find potential application as a therapeutic for Huntington's disease. The original parental mouse was used in one such study as an agonist to promote neuronal cell survival by activating the receptor and down-stream signaling proteins (PMID: 24503862).
Short Description
This chimeric mouse antibody was made using the variable domain sequences of the original Human IgG1 format, for improved compatibility with existing reagents, assays and techniques.
AB03000-3.3-BT Datasheet
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