Anti-FLT3 [EB10]
Catalogue Number: AB03003-23.0-ABA
| Manufacturer: | Vector Laboratories, Inc (ABA) |
| Type: | Recombinant Monoclonal |
| Alias: | FLK-2; FLT-3; STK-1; CD135; Fetal liver kinase 2; FL cytokine receptor; stem cell tyosine kinase 1; Fms-like tyrosine kinase 3; Receptor-type tyrosine-protein kinase FLT3 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 200 ug |
| Host name: | Rabbit |
| Clone: | EB10 |
| Isotype: | IgG |
| Immunogen: | The original Fab antibody was isolated from a human Fab phage display by selection of a high affinity antibody binding to the human FLT3. |
| Application: | ELISA, FC, SPR |
Additional Text
Gene ID
2322
Gene Name
FLT3
Uniprot ID
P36888
Purification
Purified
Antibody Clonality
Recombinant Monoclonal
Storage Note
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Application Notes
The clone was shown to selectively bind to human FLT3 with high affinity, block FL binding and mediated potent anti-tumor activity in xenograft model by a mechanism involving activation of immune effector function. The ability of the antibody to bind the receptor was examined by ELISA (EC50 within the range 0.5-1 nM). The ability of the antibody to block receptor-ligand binding was examined in a FL binding competition ELISA, the antibody blocked the binding of FL to FLT3 with a IC50 within the range of 0.5-1 nM. The clone binds FLT3 with an affinity of 0.158 nM as determined by surface plasmon resonance (SPR). Flow cytometric analysis showed that the antibody bound to the wild-type FLT-3 expressed on EOL-1 cells and also to ITD-mutant FLT3 expressed on BaF3-ITD cells (US8071099B2).
Short Description
This chimeric rabbit antibody was made using the variable domain sequences of the original Human Fab format for improved compatibility with existing reagents assays and techniques.
AB03003-23.0 Datasheet
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