Anti-Botulinum neurotoxin [BotFab 5]

Catalogue Number: AB03006-23.0-ABA

Manufacturer:	Vector Laboratories, Inc (ABA)
Type:	Recombinant Monoclonal
Alias:	BOTOX; BoNT/A; Bontoxilysin-A; Botulinum neurotoxin type A1; Botulinum neurotoxin type A; BoNT/B; Bontoxilysin-B; Botulinum neurotoxin type B; botulinum types A and B toxin complexes.
Shipping Condition:	Blue Ice
Unit(s):	200 ug
Host name:	Rabbit
Clone:	BotFab 5
Isotype:	IgG
Immunogen:	The original antibody was generated by panning the library against botulinum type B complex.
Application:	ELISA, FC, SPR

Overview
Datasheets

Additional Text

Purification

Purified

Gene ID

5185061

Antibody Clonality

Recombinant Monoclonal

Gene Name

bont

Storage Note

Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.

Uniprot ID

P0DPI0, P10844

Application Notes

The Fab was shown by ELISA to be reactive to botulinum types A and B toxin complexes and not reactive to the 150 kDa NT of type A or B that lacked the non-NT proteins found in the complex. (US5932449A) Surface plasmon resonance demonstrated that the Fab displays a slightly tighter equilibrium binding constant towards botulinum toxin B than toxin A. The dissociation rate constants (kd1 and kd2) with botulinum toxin A are 7.1x10^-3 and 2.3x10^-4 s^-1 (ratio 1:2), while with botulinum toxin B the values are 3.7x10^-3 and 5.7x10^-4 s^-1 (ratio 1:3.5). The Fab antibody was used in an indirect flow cytometric immunoassay to detect the presence of pentavalent toxoid A-B-C-D-E and botulinum toxoid A; the antibody detected the presence of botulinum toxoid A. The limit of sensitivity of the Fab fragment for botulinum toxoid is between 5 and 10 ng/ml (PMID: 10945449)

Short Description

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse Fab format for improved compatibility with existing reagents assays and techniques.

AB03006-23.0 Datasheet

Price

£390.00

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