Anti-Botulinum neurotoxin [BotFab 5]
Catalogue Number: AB03006-23.0-BT-ABA
| Manufacturer: | Vector Laboratories, Inc (ABA) |
| Type: | Recombinant Monoclonal |
| Alias: | BOTOX; BoNT/A; Bontoxilysin-A; Botulinum neurotoxin type A1; Botulinum neurotoxin type A; BoNT/B; Bontoxilysin-B; Botulinum neurotoxin type B; botulinum types A and B toxin complexes. |
| Shipping Condition: | Blue Ice |
| Unit(s): | 1 mg |
| Host name: | Rabbit |
| Clone: | BotFab 5 |
| Isotype: | IgG |
| Immunogen: | The original antibody was generated by panning the library against botulinum type B complex. |
| Application: | ELISA, FC, SPR |
Additional Text
Purification
Purified
Gene ID
5185061
Antibody Clonality
Recombinant Monoclonal
Gene Name
bont
Uniprot ID
P0DPI0, P10844
Storage Note
Store at 4⁰C for up to 3 months. Note, this antibody is provided without added preservatives, it is therefore recommed this antibody be handled under sterile conditions. For longer storage, aliquot and store at -20⁰C.
Application Notes
The Fab was shown by ELISA to be reactive to botulinum types A and B toxin complexes and not reactive to the 150 kDa NT of type A or B that lacked the non-NT proteins found in the complex. (US5932449A) Surface plasmon resonance demonstrated that the Fab displays a slightly tighter equilibrium binding constant towards botulinum toxin B than toxin A. The dissociation rate constants (kd1 and kd2) with botulinum toxin A are 7.1x10^-3 and 2.3x10^-4 s^-1 (ratio 1:2), while with botulinum toxin B the values are 3.7x10^-3 and 5.7x10^-4 s^-1 (ratio 1:3.5). The Fab antibody was used in an indirect flow cytometric immunoassay to detect the presence of pentavalent toxoid A-B-C-D-E and botulinum toxoid A; the antibody detected the presence of botulinum toxoid A. The limit of sensitivity of the Fab fragment for botulinum toxoid is between 5 and 10 ng/ml (PMID: 10945449)
Short Description
This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse Fab format for improved compatibility with existing reagents assays and techniques.
AB03006-23.0-BT Datasheet
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