Anti-Rabbit IgG [A10B]

Catalogue Number: AB03007-24.1-BT-ABA

Manufacturer:	Vector Laboratories, Inc (ABA)
Type:	Recombinant Monoclonal
Alias:	immunoglobulin gamma
Shipping Condition:	Blue Ice
Storage Condition:	2-8°C
Unit(s):	500 ug
Host name:	Goat
Clone:	A10B
Isotype:	IgG
Immunogen:	The antibody was originally generated by immunizing BALB/c mice with purified rabbit IgG.
Application:	ELISA, WB, SPR

Overview
Datasheets

Additional Text

Purification

Purified

Antibody Clonality

Recombinant Monoclonal

Storage Note

Store at 4⁰C for up to 3 months. Note, this antibody is provided without added preservatives, it is therefore recommed this antibody be handled under sterile conditions. For longer storage, aliquot and store at -20⁰C.

Application Notes

Different formats of the antibody (scFv-cys, scFv, Fab and IgG) were employed for the construction of biosensors used for antigen detection in complex samples. In scFv-cys, one glycine of the linker was replaced with a cysteine, which determine an orientation of the scFv fragments on the sensor surface. The performance, in terms of sensitivity and selectivity, in biosensors prepared with scFv-cys, scFv, Fab and IgG was compared. The scFv-cys based biosensors displayed greater assay sensitivity and exhibited less nonspecific activity than intact monoclonal IgG and Fab fragment-based immunosensors. The scFv-cys biosensor surface was easily regenerated by stripping the rabbit IgG from the surface using an acidic solution. Moreover, western blot analysis showed purified scFv migrated as a single band at a MW of ~32 000 and did not appear to be degraded and the specificity of IgG1, Fab fragment and scFv towards rabbit IgG was determined by ELISA. (PMID: 15679346) In another study, two histidine amino acids were incorporated into the linker peptide of the scFv fragment and the scFv-His fragment was then employed to develop a new biosensor. The specificity of scFv and scFv-Hys were confirmed by ELISA. (PMID: 16255580) The affinity constant of rabbit IgG binding with scFv measured by using a conventional regeneration SPR method ((2.2 +/- 1.5) x 10^7 M-1) was comparable with the value determined with a nonregeneration protocol (2.5 +/- 0.2) x 10^7 M-1, and quartz crystal microbalance (1.9 x 10^7 M-1). (PMID: 16536419)

Short Description

This chimeric goat antibody was made using the variable domain sequences of the original rabbit format, for improved compatibility with existing reagents, assays and techniques.

AB03007-24.1-BT Datasheet

Price

£2,190.00

-

Quantity
Unit Size

Find out about delivery