Anti-HA cleavage site [A3]
Catalogue Number: AB03021-23.0-ABA
| Manufacturer: | Vector Laboratories, Inc (ABA) |
| Type: | Recombinant Monoclonal |
| Alias: | Hemagglutinin Cleavage Site; CS HA; Avian Influenza Virus; H5N1 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 200 ug |
| Host name: | Rabbit |
| Clone: | A3 |
| Isotype: | IgG |
| Immunogen: | The original antibody was generated by immunizing mice with the cleavage site (CS) peptide of hemagglutinin protein, followed by antibody library construction and phage display-based selection. |
| Application: | ELISA, IF, SPR |
Additional Text
Purification
Purified
Antibody Clonality
Recombinant Monoclonal
Storage Note
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Uniprot ID
A5A5M5
Application Notes
The specificity of this antibody (Fab) was confirmed by ELISA. The epitope mapping of this antibody was done using ELISA. The binding affinity of the Fab fragment towards the cleavage site peptide of HA derived from the HPAI H5N1 virus was found to be Kd= 0,80 nM using surface plasmon resonance. The clone was used for detection of the cleavage site peptide on an open sandwich ELISA, reaching the lower limit of detection (LOD) in the range of 1 to 10 ng/ml. This antibody was also used in the immunofluorescent staining of Madin-Darby canine kidney cells infected with HPAI H5N1 (Dong et al, 2013; pmid:23577205).
Short Description
This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse Fab format, for improved compatibility with existing reagents, assays and techniques.
AB03021-23.0 Datasheet
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