Anti-VP35 [H3]
Catalogue Number: AB03025-15.0-ABA
| Manufacturer: | Vector Laboratories, Inc (ABA) |
| Type: | Recombinant Monoclonal |
| Alias: | Polymerase cofactor VP35; VP35; mVP35; Marburg VP35; Marburg virus protein 35; sFab H3 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 50 ug |
| Host name: | Human |
| Clone: | H3 |
| Isotype: | IgM |
| Immunogen: | The original antibody was generated by using phage display technology. The library was constructed on a single human Fab framework with variable domains from subgroups VH3 and Vκ1. The synthetic Fab phage library was screened against the recombinant mVP35 IFN inhibitory domain (IID) protein of Marburg virus. |
| Application: | ELISA, Inh, Crstapy |
Additional Text
Purification
Purified
Antibody Clonality
Recombinant Monoclonal
Storage Note
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
UniProt ID
Q03039
Application Notes
The specificity of this antibody (Fab) was confirmed by ELISA analysis. The antibody was crystallized with the antigen. The dissociation constants (KD) measured using isothermal-titration calorimetry (ITC) revealed that the antibody binds to mVP35 IID with high affinity (KD = 4.90 +/- 1 nM) and it exhibited 1:1 stoichiometry. The scFv format of the antibody was employed to test if the antibody binding interferes with mVP35 function in viral replication, scFv expression inhibited the Marburg polymerase complex in a dose-dependent manner but did not inhibit Ebola minigenome activity (Amatya et al, 2019; pmid:31120240).
Short Description
This reformatted human antibody was made using the variable domain sequences of the original Human Fab format, for improved compatibility with existing reagents, assays and techniques.
AB03025-15.0 Datasheet
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