Anti-S2 domain [S2A3]

Catalogue Number: AB03026-23.0-BT-ABA

Manufacturer:Vector Laboratories, Inc (ABA)
Type:Recombinant Monoclonal
Alias:MERS-S2P; MERS-CoV S2 subunit; Spike protein S2; Spike glycoprotein; S glycoprotein; E2; Peplomer protein
Shipping Condition:Blue Ice
Unit(s): 1 mg
Host name: Rabbit
Clone: S2A3
Isotype: IgG
Immunogen: The original antibody was generated from a human Fab phage display library panned against the S2 subunit of the MERS-CoV spike protein (MERS-S2P).
Application: ELISA, IF, SPR

Additional Text

Purification

Purified

Antibody Clonality

Recombinant Monoclonal

Storage Note

Store at 4⁰C for up to 3 months. Note, this antibody is provided without added preservatives, it is therefore recommed this antibody be handled under sterile conditions. For longer storage, aliquot and store at -20⁰C.

UniProt ID

W6A028

Application Notes

The specificity of this antibody (Fab) was confirmed by ELISA analysis. Immunofluorescence was performed on Vero cells using this antibody (IgG). The binding affinity of the IgG antibody towards MERS-S2P was found to be Kd= 170 pM using surface plasmon resonance. The antibody (IgG) was used as a capture antibody in sandwich ELISA (Kim et al, 2019; pmid: 31544848).

Short Description

This chimeric rabbit antibody was made using the variable domain sequences of the original Human Fab, IgG format, for improved compatibility with existing reagents, assays and techniques.