Anti-S protein (RBD) [5F8]
Catalogue Number: AB03059-10.159-ABA
| Manufacturer: | Vector Laboratories, Inc (ABA) |
| Type: | Recombinant Monoclonal |
| Shipping Condition: | Blue Ice |
| Unit(s): | 0.1 mg |
| Host name: | Human |
| Clone: | 5F8 |
| Isotype: | IgG1 |
| Immunogen: | The original antibody was generated by using phage display technology by biopanning a full synthetic, humanized phage display library with recombinant RBD protein. |
| Application: | SPR, InVitroNT |
Additional Text
Gene Name
S
Purification
Purified
Antibody Clonality
Recombinant Monoclonal
Gene ID
43740568
Uniprot ID
P0DTC2
Storage Note
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Specificity
Spike glycoprotein; Receptor Binding Domain; SARS CoV 2 S glycoprotein; COVID-19 Spike protein; RBD; Receptor Binding Domain; S glycoprotein; SARS coronavirus 2 S protein; SARS coronavirus 2 Spike Protein; SARS CoV 2 Spike protein; SARS CoV 2; SARS-CoV-2 S protein; SARSCoV2; SARS-COV-2 S protein; SARS-COV-2 Spike glycoprotein; SARSCOV2 Spike protein; Severe acute respiratory syndrome 2 spike glycoprotein; Severe acute respiratory syndrome virus 2 spike glycoprotein; S glycoprotein; E2; Peplomer protein
Application Notes
The binding affinity of the VHH fragment towards SARS-CoV-2 or SARC-CoV RBD was measured using surface plasmon resonance (Kd= 0.996 and 239,2 nM respectively). The neutralization activity of the VHH fragment with SARS-CoV-2 pseudotypes or SARS-CoV-2 was tested, the showing half maximal neutralization concentration (EC50) of 0.0009 µg/mL and 0.51 µg/mL respectively. Competition-binding assay showed the clone could just partially prevent binding of SARS-CoV-2 RBD to ACE2. Neutralization assay of the bivalent form of the clone fused with human IgG1 Fc against SARS-CoV-2pp was performed, showing EC50= 0.004 µg/mL (Chi et al, 2020; pmid: 32913273).
Short Description
This chimeric human antibody was made using the variable domain sequences of the original VHH format for improved compatibility with existing reagents assays and techniques.
AB03059-10.159 Datasheet
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