Anti-S protein (RBD) [5F8]

Catalogue Number: AB03059-10.159-BT-ABA

Manufacturer:	Vector Laboratories, Inc (ABA)
Type:	Recombinant Monoclonal
Shipping Condition:	Blue Ice
Unit(s):	0.5 mg
Host name:	Human
Clone:	5F8
Isotype:	IgG1
Immunogen:	The original antibody was generated by using phage display technology by biopanning a full synthetic, humanized phage display library with recombinant RBD protein.
Application:	SPR, InVitroNT

Overview
Datasheets

Additional Text

Gene Name

Purification

Purified

Antibody Clonality

Recombinant Monoclonal

Gene ID

43740568

Uniprot ID

P0DTC2

Specificity

Spike glycoprotein; Receptor Binding Domain; SARS CoV 2 S glycoprotein; COVID-19 Spike protein; RBD; Receptor Binding Domain; S glycoprotein; SARS coronavirus 2 S protein; SARS coronavirus 2 Spike Protein; SARS CoV 2 Spike protein; SARS CoV 2; SARS-CoV-2 S protein; SARSCoV2; SARS-COV-2 S protein; SARS-COV-2 Spike glycoprotein; SARSCOV2 Spike protein; Severe acute respiratory syndrome 2 spike glycoprotein; Severe acute respiratory syndrome virus 2 spike glycoprotein; S glycoprotein; E2; Peplomer protein

Storage Note

Store at 4⁰C for up to 3 months. Note, this antibody is provided without added preservatives, it is therefore recommed this antibody be handled under sterile conditions. For longer storage, aliquot and store at -20⁰C.

Application Notes

The binding affinity of the VHH fragment towards SARS-CoV-2 or SARC-CoV RBD was measured using surface plasmon resonance (Kd= 0.996 and 239,2 nM respectively). The neutralization activity of the VHH fragment with SARS-CoV-2 pseudotypes or SARS-CoV-2 was tested, the showing half maximal neutralization concentration (EC50) of 0.0009 µg/mL and 0.51 µg/mL respectively. Competition-binding assay showed the clone could just partially prevent binding of SARS-CoV-2 RBD to ACE2. Neutralization assay of the bivalent form of the clone fused with human IgG1 Fc against SARS-CoV-2pp was performed, showing EC50= 0.004 µg/mL (Chi et al, 2020; pmid: 32913273).

Short Description

This chimeric human antibody was made using the variable domain sequences of the original VHH format for improved compatibility with existing reagents assays and techniques.

AB03059-10.159-BT Datasheet

Price

£2,215.00

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