Anti-NP [JS16]
Catalogue Number: AB03061-23.0-ABA
| Manufacturer: | Vector Laboratories, Inc (ABA) |
| Type: | Recombinant Monoclonal |
| Alias: | N; Nucleoprotein; Nucleocapsid protein; NC; Protein N |
| Shipping Condition: | Blue Ice |
| Unit(s): | 200 ug |
| Host name: | Rabbit |
| Clone: | JS16 |
| Isotype: | IgG |
| Immunogen: | A combinatorial fragment of antigen-binding (Fab)antibody phage library based on peripheral blood-derived from five coronavirus disease 2019 (COVID-19) infected donors was constructed. The Fab antibody was obtained and identified by panning with purified NP. |
| Application: | ELISA, WB, SPR |
Additional Text
Gene Name
N
Purification
Purified
Antibody Clonality
Recombinant Monoclonal
Gene ID
43740575
Uniprot ID
P0DTC9
Storage Note
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Application Notes
The specificity of the Fab and the IgG1 formats for SARS-CoV-2 NP was confirmed by ELISA analysis. The IgG1 antibody detected SARA-Cov-2 NP by western blot. Surface plasmon resonance assay was performed to evaluate the binding specificity and affinity between antibody and purified NP; the antibody showed a tight binding affinity to immobilized NP. The antibody was used as a capture antibody in sandwich ELISA, where it was used in a matched pair in conjunction with JS08, with the optimal detection limit below 0.78ng/mL. The epitope of the antibody was analyzed by competition ELISA (Zhang et al, 2020; pmid:33281824).
Short Description
This chimeric rabbit antibody was made using the variable domain sequences of the original Human Fab format for improved compatibility with existing reagents assays and techniques.
AB03061-23.0 Datasheet
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