Anti-NKG2D [KYK-2]
Catalogue Number: AB03075-2.0-ABA
| Manufacturer: | Vector Laboratories, Inc (ABA) |
| Type: | Recombinant Monoclonal |
| Alias: | KLRK1; CD314; NKG2-D type II integral membrane protein; NK cell receptor D; Killer cell lectin-like receptor subfamily K member 1; NKG2-D-activating NK receptor; KYK2; KYK-2.0 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 200 ug |
| Host name: | Mouse |
| Clone: | KYK-2 |
| Isotype: | IgG2a |
| Immunogen: | The original antibody was isolated from a Fab library generated from the bone marrow from 6 healthy donors by collecting total RNA preparation and RT-PCR amplification of human Vκ, Vλ, and VH encoding sequences. The library was panned against human NKG2D for isolation of this clone. |
| Application: | ELISA, FC, Agon, Ant |
Additional Text
Uniprot ID
P26718
Purification
Purified
Gene ID
100528032; 22914
Gene Name
KLRC4-KLRK1, KLRK1
Antibody Clonality
Recombinant Monoclonal
Storage Note
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Application Notes
The binding of this antibody to NKG2D expressed on subpopulations of human peripheral blood mononuclear cells (PBMC) was done using flow cytometry. This antibody was not capable of binding mouse NKG2D. The IgG1 version of this antibody is reported to show dual antagonistic and agonistic activity. It was reported that soluble KYK-2.0 IgG1 exhibits antagonistic activity through interfering with effector cell to target cell recognition mediated by NKG2D receptor/ligand interactions. KYK-2.0 IgG1 in solution significantly blocked the acquired cytolytic activity of ex vivo expanded human NK cells. The agonistic activity of this antibody was determined using a degranulation assay. It was shown that immobilized KYK-2.0 IgG1 potently induced NK cell degranulation in PBMC from 4 different healthy donors (PMID: 18809410). This antibody was also used in the generation of a bispecific antibody targeting NKG2D and 2B4 receptors. The combination mounts selective cytotoxicity and IFN-γ production of NK cells and helps in assessing NK cell functions (PMID: 34486371). This antibody was also used in the generation of another bispecific antibody against KNG2B and ErbB2 (PMID: 34599028).
Short Description
This chimeric mouse antibody was made using the variable domain sequences of the original Human Fab format for improved compatibility with existing reagents assays and techniques.
AB03075-2.0 Datasheet
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