Anti-LDL Receptor [C7]

Catalogue Number: AB03080-10.3-ABA

Manufacturer:	Vector Laboratories, Inc (ABA)
Type:	Recombinant Monoclonal
Alias:	LDLR; Low-density lipoprotein receptor; IgG-C7
Shipping Condition:	Blue Ice
Unit(s):	200 ug
Host name:	Human
Clone:	C7
Isotype:	IgG1
Immunogen:	The original antibody was produced by immunization of BALB/C mice with the extra cellular domain of the bovine LDL-R.
Application:	FC, IF, IP, WB, Inh

Overview
Datasheets

Additional Text

Gene Name

LDLR

Gene ID

3949

Uniprot ID

P01130

Purification

Purified

Antibody Clonality

Recombinant Monoclonal

Storage Note

Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.

Application Notes

The antibody (IgG2b) was employed for the staining of the LDL receptor in normal fibroblast. The antibody competed with LDL for binding to the LDL receptor of intact fibroblast; complete inhibition was achieved at 37 C but not at 4 C (Beisiegel et al., 1981; PMID: 6271765). The antibody detected LDL receptor from human fibroblasts and bovine adrenal cortex by western blot analysis (Beisiegel et al., 1982; PMID: 6290495). The LDL receptor was immunoprecipitated using this antibody (Davis et al., 1987; PMID: 3494949). This antibody was used for detection of LDL receptor expressed on Chang and CHO cells by flow cytometry (Holst et al., 2001; PMID: 11781697) (Ranheim et al., 2006; PMID: 16740646). Immunofluorescence was performed on liver Chang cells using this antibody, showing that the WT protein was localised on the cell surface whereas the two mutant proteins were localised intracellularly (Holst et al., 2001; PMID: 11781697). The antibody was used to inhibit the binding of LDL to its receptor LDLR in brain capillary endothelial cells. Coincubations of LDL with increasing concentrations of the antibody decreased the rate of passage of the LDL through the monolayer; LDL transcytosis was totally abolished at the concentration of 1 mg/ml of antibody. This confirmed that the LDL receptor is involved receptor-mediated transcytosis (Dehouck et al., 1997; PMID: 9265653).

Short Description

This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG2b format for improved compatibility with existing reagents assays and techniques.

AB03080-10.3 Datasheet

Price

£390.00

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