Anti-Glycoprotein GPIIb/IIIa complex [314.3]
Catalogue Number: AB03113-10.3-ABA
| Manufacturer: | Vector Laboratories, Inc (ABA) |
| Type: | Recombinant Monoclonal |
| Alias: | Integrin beta-3; Platelet membrane glycoprotein IIIa; GPIIIa; CD61; integrin αIIbβ3 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 200 ug |
| Host name: | Human |
| Clone: | 314.3 |
| Isotype: | IgG1 |
| Immunogen: | The original antibody was generated by immunizing BALB/c mice with a mixture of quinine-linked GPIIb/IIIa, unlinked GPIIb/IIIa, and soluble quinine. |
| Application: | FC, InVitroA, Crstion |
Additional Text
Gene Name
ITGB3
Gene ID
3690
Uniprot ID
P05106
Purification
Purified
Antibody Clonality
Recombinant Monoclonal
Storage Note
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Application Notes
The antibody reacted with normal human platelets in the presence of quinine, as shown by flow cytometry. The reactions of the antibody with CHO cells expressing all-human GPIIb/IIIa (H/H), all-rat GPIIb/IIIa (R/R), or human/rat mixed GPIIb/IIIa integrins (H/R, R/H) were studied by flow cytometry. Quinine dependent antibody binding occurred with constructs H/H and H/R, but not with R/R or R/H (Bougie et al., 2009; PMID: 18948570). The crystal structures of the Fab fragment and its complex with quinine were determined. Quinine induced structural modifications of the Fab fragment; the authors propose that quinine remodels the Fab paratope and enables binding to the platelet GPIIb/IIIa. The monovalent KD values for quinine binding to the IgG fragment measured was 24 nM (Zhu et al., 2015; PMID: 26282540).
Short Description
This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format for improved compatibility with existing reagents assays and techniques.
AB03113-10.3 Datasheet
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