Anti-XCR1 [MARX10]
Catalogue Number: AB03120-23.0-BT-ABA
| Manufacturer: | Vector Laboratories, Inc (ABA) |
| Type: | Recombinant Monoclonal |
| Alias: | mXCR1; Ccxcr1; Chemokine XC receptor 1; Lymphotactin receptor; SCM1 receptor; XC chemokine receptor 1; MARX1O |
| Shipping Condition: | Blue Ice |
| Unit(s): | 1 mg |
| Host name: | Rabbit |
| Clone: | MARX10 |
| Isotype: | IgG |
| Immunogen: | The original antibody was generated by immunizing B6.XCR1-lacZ mice every 3 weeks (4 times in succession) with 30 x 10^6 CD11c-enriched cells from C57BL/6 WT mice in combination with heat-inactivated B. pertussis. |
| Application: | FC, IHC, InVivoA |
Additional Text
Purification
Purified
UniProt ID
Q9R0M1
Antibody Clonality
Recombinant Monoclonal
Storage Note
Store at 4⁰C for up to 3 months. Note, this antibody is provided without added preservatives, it is therefore recommed this antibody be handled under sterile conditions. For longer storage, aliquot and store at -20⁰C.
Short Description
This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2b format for improved compatibility with existing reagents assays and techniques.
Application Notes
This antibody was used to stain cryostat sections (12 µm) of spleens from C57BL/6 WT and B6.XCR1-lacZ mice. It was found that this antibody could stain 20% of splenic conventional dendritic cells in C57BL/6 WT mice but did not give a signal in homozygous B6.XCR1-lacZ animals, which lack the XCR1 receptor. This antibody was also used to subsets of dendritic cells that express XCR1 using flow cytometry (Bachem et al., 2012; PMID: 22826713). Digoxigenin (Dig) coupled MARX10 was injected into C57BL/6 mice for in vivo labeling of XCR1+ dendritic cells. Recombinant ovalbumin (OVA) coupled MARX10 (MARX10-OVA) was used to measure antibody levels in C57BL/6 and homozygous B6.XCR1-lacZ mice serum following injection and for an in vivo cytotoxicity assay; MARX10-OVA was found to be highly effective in inducing CD8+ T cell cytotoxicity in vivo (Hartung et al., 2015; PMID: 25520399). This antibody has been added to the Antibody Registry (RRID: AB_3668949).
AB03120-23.0-BT Datasheet
SUPPORT
outstanding technical support
PRODUCT
we offer a full product guarantee
DELIVERY
we offer free delivery to UK universities and non profit organisations