Anti-CD20 [NCD1.2 (scFv-3)]
Catalogue Number: AB03144-202.1-BT-ABA
| Manufacturer: | Vector Laboratories, Inc (ABA) |
| Type: | Recombinant Monoclonal |
| Alias: | Bp35; Leu-16; MS4A1; Leukocyte surface antigen Leu-16; B-lymphocyte antigen CD20; B-lymphocyte surface antigen B1; Membrane-spanning 4-domains subfamily A member 1 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 1 mg |
| Host name: | Dog |
| Clone: | NCD1.2 (scFv-3) |
| Isotype: | IgG4 |
| Immunogen: | The original antibody was generated by immunizing 5-8 weeks old BALB/c mice with a canine CD20 peptide (NCDPANPSEKNSLSIQYCGS) at a final concentration of 100 µg/ml in complete Freud's adjuvant. |
| Application: | FC, IF, WB, IHC |
Additional Text
Gene Name
MS4A1
Purification
Purified
Antibody Clonality
Recombinant Monoclonal
Gene ID
485430; 931
Uniprot ID
P11836, Q3C2E2
Storage Note
Store at 4⁰C for up to 3 months. Note, this antibody is provided without added preservatives, it is therefore recommed this antibody be handled under sterile conditions. For longer storage, aliquot and store at -20⁰C.
Application Notes
The original IgG antibody binds biotinylated canine CD20 peptide with an relative affinity of kd= 340 pM. This antibody was able to recognize endogenous canine CD20 protein in canine 3132 lymphoma cells and also cross reacted with transfected human GFP-CD20 expression construct in H1299 cells in an western blot assay. This antibody can detect canine CD20 expressed on normal peripheral blood, medium-sized B-cell lymphoma and diffuse large B-cell lymphoma in a flow cytometric assay. It could not detect CD20 -negative peripheral T-cell lymphoma cells in flow cytometry. This antibody was also used in the immunohistochemical staining of formalin fixed samples of diffuse large B-cell lymphoma and lymphoma histotypes of B-cell origin, including marginal zone lymphoma, follicular lymphoma, and small lymphocytic lymphoma. (PMID: 26894679)
Short Description
This chimeric dog antibody was made using the variable domain sequences of the original Mouse IgG2a format, for improved compatibility with existing reagents, assays and techniques.
AB03144-202.1-BT Datasheet
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