Anti-Lipid A [S1-15]
Catalogue Number: AB03158-3.0-ABA
| Manufacturer: | Vector Laboratories, Inc (ABA) |
| Type: | Recombinant Monoclonal |
| Alias: | LIPA |
| Shipping Condition: | Blue Ice |
| Unit(s): | 200 ug |
| Host name: | Mouse |
| Clone: | S1-15 |
| Isotype: | IgG2b |
| Immunogen: | The original antibody was generated by immunizing BALB/c mice with lipA. |
| Application: | ELISA, EIA, Crstion |
Additional Text
Purification
Purified
Antibody Clonality
Recombinant Monoclonal
Storage Note
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Application Notes
In passive immunohemolysis, the antibody reacted with bisphosphoryl compounds as well as with the 4'-monophosphoryl partial structures (Kuhn et al., 1992; PMID 1375194). The specificity of the antibody was determined by ELISA analysis. The crystal structures of the Fab fragment in complex with lipid A carbohydrate backbone and in the unliganded form were determined by X-ray diffraction, showing the binding mechanism of the antibody is a lock and key type. Further, the structure of the Fab fragment in complex with single-stranded DNA fragment 5(dT) oligonucleotide was determined. The antibody bound two separate oligonucleotides by their terminal 5- and 3-phosphate groups (Haji-Ghassemi et al., 2015; PMID: 26085093). Solid-phase enzyme immunoassay shown the antibody binds better to the glycoconjugate at low antigen concentrations and better to the lipid A when high concentrations of antigen were used (Brade et al., 1993). The antibody was tested against the synthetic lipid A precursor Ia and compound B1047, a precursor Ia, by enzyme immunoassay. The antibody bound to precursor Ia, but not to compound B 1047 or Escherichia coli Re LPS (Brade et al., 1997; PMID: 9284181).
AB03158-3.0 Datasheet
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